Optical sectioning by two-pinhole confocal fluorescence microscopy
نویسندگان
چکیده
منابع مشابه
Optimal detection pinhole for lowering speckle noise while maintaining adequate optical sectioning in confocal reflectance microscopes.
Coherent speckle influences the resulting image when narrow spectral line-width and single spatial mode illumination are used, though these are the same light-source properties that provide the best radiance-to-cost ratio. However, a suitable size of the detection pinhole can be chosen to maintain adequate optical sectioning while making the probability density of the speckle noise more normal ...
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ژورنال
عنوان ژورنال: Micron
سال: 2003
ISSN: 0968-4328
DOI: 10.1016/s0968-4328(03)00057-x